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EMF Study
(Database last updated on Dec 1, 2021)

ID Number 2076
Study Type In Vitro
Model Temperature detection from RF exposure in animals, rat brain tissue, and cell culture using Rhodamine dyes.

Biological samples were treated with the fluorescent dye Rhodamine B. RF-induced temperature was shown to cause a shift in fluorescence as detected by laser scanning confocal microscopy of tissue samples. Detection had a spatial resolution of a few microns and corresponded to temperature readings with fiber optic probes with an accuracy of 1.5°C and a reproducibility of +/- 6%. AUTHORS' ABSTRACT: Bermingham, CHen, McIntosh and Wood 2014 (IEEE #5416): Fluorescent intensity of the dye Rhodamine-B (Rho-B) decreases with increasing temperature. We show that in fresh rat brain tissue samples in a custom-made radiofrequency (RF) tissue exposure device, temperature rise due to RF radiation as measured by absorbed dye correlates well with temperature measured nearby by fiber optic probes. Estimates of rate of initial temperature rise (using both probe measurement and the dye method) accord well with estimates of local specific energy absorption rate (SAR). We also modeled the temperature characteristics of the exposure device using combined electromagnetic and finite-difference thermal modeling. Although there are some differences in the rate of cooling following cessation of RF exposure, there is reasonable agreement between modeling and both probe measurement and dye estimation of temperature. The dye method also permits measurement of regional temperature rise (due to RF). There is no clear evidence of local differential RF absorption, but further refinement of the method may be needed to fully clarify this issue.

Findings Not Applicable to Bioeffects
Status Completed With Publication
Principal Investigator Swinburne University of Technology, Australia -
Funding Agency ACEBR, Australia
  • Chen, YY et al. Bioelectromagnetics., (2009) 30:583-590
  • Bermingham, JF et al. Bioelectromagnetics., (2014) 35:181-191
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