Peripheral blood was isolated from volunteers (n = 60) both before and after mobile phone use for 30 minutes and compared for differences in cellular immunity indicators (i.e. CD4+CD45RA+ naive cells, CD4+CD45RO+ memory cells, CD4+ helper cells, CD8+ cytotoxic cells, CD19+ B lymphocytes, CD3-CD16+CD56+natural killer cells, total (CD3+) T cells) using flow cytometry. In addition, cytokines (TNF-alpha, IL-1beta, IL-6) were determined from peripheral blood cells cultured for 48 hours using ELISA. A subsequent study using 32 female volunteers using a similar study design to look at immune and cytokine endpoints was also performed. The authors preliminarily report that mobile phone exposure correlated with a significant increase in CD4+CD45RO+ memory cells in females (p<0.05) and CD4+ helper cells in males and females (p<0.01 and p<0.001, respectively). In contrast, mobile phone exposure correlated with a decrease in CD3- CD16+ CD56+ natural killer cells in males and females (p<0.01), CD19+ B lymphocytes in females (p<0.01), and total lymphocytes in males (p<0.05). Decreases in CD8+ cytotoxic cells were significantly associated with smoking (p<0.01), drinking (p<0.01), and daily use of mobile phones (p<0.01). Mobile phone exposure was also reported to decrease IL-1 beta and IL-6 in lymphocytes isolated from both males and females (p<0.01). Interleukin-6 concentration was significantly associated with dizziness (r=0.658, p<0.01) and discomfort (r=-0.533, p<0.05) after wireless phone use. Interleukin-1 concentration was associated with average amount of drinking (r=0.594, p<0.01) and eye itching and tear dripping (r=0.477, p<0.01). Other correlations between immune cell levels and subjective disorder complaints were also reported.